Raw Data for BglB Variant N157Q

Expression Data

Yield (mg/mL): 0.119

Thermostability Assay Data

slope of N157Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	5.00E-03	5.00E-03	4.50E-03
	B	31.3	6.00E-03	6.00E-03	5.50E-03
	C	33.6	6.50E-03	6.50E-03	5.50E-03
	D	37.7	6.00E-03	7.00E-03	6.50E-03
	E	42.4	2.00E-03	2.00E-03
	F	45.7		5.00E-04	5.00E-04
	G	48.3	5.00E-04	5.00E-04	5.00E-04
	H	50.0	0.00E+00		0.00E+00

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:15:10.

Reference Data for WT

slope of N157Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	5.70E-02	5.70E-02	5.80E-02
	B	31.3	5.80E-02	5.65E-02	5.55E-02
	C	33.6	5.80E-02	6.20E-02	6.00E-02
	D	37.7	4.65E-02	4.50E-02	4.70E-02
	E	42.4	1.80E-02	1.65E-02	1.75E-02
	F	45.7		5.00E-04	5.00E-04
	G	48.3		5.00E-04	5.00E-04
	H	50.0		7.40E-17	0.00E+00

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:09:23.

Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.