Raw Data for BglB Variant L171N

Expression Data

SDS-PAGE
 0.258

Kinetic Assay Data

slope of L171N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.60E-02 3.40E-02 3.50E-02
B 25.00 2.60E-02 2.55E-02 2.50E-02
C 8.33 1.70E-02 1.70E-02 1.65E-02
D 2.78 1.00E-02 1.00E-02 1.00E-02
E 0.93 5.00E-03 5.00E-03 4.50E-03
F 0.31 1.50E-03 1.50E-03 1.50E-03
G 0.10 1.00E-03 1.00E-03
H 0.03 0.00E+00 0.00E+00
 0.189706      100×

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by joshuaraychen and last updated on 2025-04-15 14:20:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.75E-02 6.35E-02
B 25.00 5.60E-02 4.35E-02 5.55E-02
C 8.33 4.10E-02 3.45E-02 4.40E-02
D 2.78 2.20E-02 1.85E-02 2.10E-02
E 0.93 9.00E-03 9.50E-03 9.00E-03
F 0.31 4.00E-03 3.00E-03 3.00E-03
G 0.10 2.50E-03 1.00E-03 1.50E-03
H 0.03 5.00E-04 5.00E-04
 0.264      100×

Protein purified on 3/4/25 and assayed on 3/25/25.
Data uploaded by DestinyMontero and last updated on 2025-04-15 14:54:55.


Thermostability Assay Data

slope of L171N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.48e-16 1.48e-16
D 42.4 2.00E-03 2.00E-03
E 37.7 1.25E-02 1.20E-02 1.10E-02
F 33.6 1.70E-02 1.60E-02 1.60E-02
G 31.3 1.80E-02 1.90E-02 1.75E-02
H 30.0 1.85E-02 1.95E-02 1.80E-02

Protein purified on 2/25/25 and assayed on 4/1/25.
Data uploaded by joshuaraychen and last updated on 2025-04-15 14:25:25.


Reference Data for WT

slope of L171N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 5.00E-04 5.00E-04
D 42.4 1.95E-02 1.85E-02
E 37.7 6.90E-02 6.95E-02 6.60E-02
F 33.6 7.30E-02 7.75E-02 7.30E-02
G 31.3 8.30E-02 1.08E-01 9.25E-02
H 30.0 7.75E-02 7.70E-02 7.55E-02

Protein purified on 3/4/25 and assayed on 4/1/25.
Data uploaded by DestinyMontero and last updated on 2025-04-15 14:17:49.


Design Data

4.392

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.