Raw Data for BglB Variant W399H

Expression Data

SDS-PAGE
 0.23

Kinetic Assay Data

slope of W399H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.50E-03 8.00E-03
B 25.00 8.00E-03 4.50E-03
C 8.33 7.00E-03 7.50E-03
D 2.78 3.50E-03 6.50E-03 1.50E-03
E 0.93 1.00E-03 1.00E-03
F 0.31 1.00E-03 1.00E-03
G 0.10 0.00E+00 0.00E+00
H 0.03 0.00E+00 0.00E+00
 0.23      10×

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by DavidS23 and last updated on 2025-04-15 14:42:01.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.60E-02 7.85E-02 7.55E-02
B 25.00 6.40E-02 6.60E-02 6.15E-02
C 8.33 4.70E-02 4.15E-02 4.80E-02
D 2.78 2.90E-02 2.90E-02 2.85E-02
E 0.93 1.35E-02 1.30E-02 1.30E-02
F 0.31 5.50E-03 5.00E-03 5.00E-03
G 0.10 2.50E-03 2.00E-03 2.00E-03
H 0.03 7.40E-17 7.40E-17
 0.1803      10×

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by ushuunamarra and last updated on 2025-04-15 14:39:54.


Thermostability Assay Data

slope of W399H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.48E-16 1.48E-16
D 42.4 1.50E-03 1.50E-03
E 37.7 1.50E-03 1.50E-03
F 33.6 2.50E-03 2.00E-03 2.00E-03
G 31.3 2.00E-03 2.00E-03 2.00E-03
H 30.0 2.00E-03 2.00E-03 2.00E-03

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by DavidS23 and last updated on 2025-04-15 13:45:21.


Reference Data for WT

slope of W399H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.00E-03 1.00E-03
D 42.4 1.90E-02 1.65E-02 1.90E-02
E 37.7 4.30E-02 3.90E-02 3.80E-02
F 33.6 4.70E-02 4.65E-02 4.40E-02
G 31.3 4.45E-02 4.45E-02 4.50E-02
H 30.0 4.55E-02 4.60E-02 4.10E-02

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by ushuunamarra and last updated on 2025-04-15 14:23:50.


Design Data

5.523

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.