Raw Data for BglB Variant H178F

Expression Data

SDS-PAGE
 0.088

Kinetic Assay Data

slope of H178F A420 nm (s−1)
[S] (mm) 1 2 3
A 75.00 0.018 0.0165
B 25.00 0.0155 0.014
C 8.33 0.012 0.012 0.0095
D 2.78 0.0015 0.009 0.0055
E 0.93 0.005 0.004
F 0.31 0.0045 0.0025 0.0015
G 0.10 0.0015 0.0015
H 0.03 0.0005 0.0005
 0.088      10×

Protein purified on 3/25/25 and assayed on 3/25/25.
Data uploaded by jessicale03 and last updated on 2025-04-15 14:43:09.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.02E-01 2.23E-01
B 25.00 3.00E-02 1.99E-01 1.40E-01
C 8.33 1.49E-01 1.77E-01
D 2.78 1.40E-02 9.10E-02 5.80E-02
E 0.93 4.40E-02 4.90E-02
F 0.31 3.00E-03 1.70E-02 1.20E-02
G 0.10 7.00E-03 6.50E-03
H 0.03 0.00E+00 5.00E-04 1.00E-03
 0.2875      10×

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:32:02.


Thermostability Assay Data

slope of H178F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0005 0.0005
C 45.7 0 1.48E-16
D 42.4 0.007 0.007
E 37.7 0.0275 0.0325 0.0295
F 33.6 0.042 0.045 0.035
G 31.3 0.0505 0.041 0.0515
H 30.0 0.0545 0.0555

Protein purified on 4/1/25 and assayed on 4/1/25.
Data uploaded by jessicale03 and last updated on 2025-04-15 14:01:19.


Reference Data for WT

slope of H178F A420 nm (min−1)
T (°C) 1 2 3
A 30.0 5.70E-02 5.70E-02 5.80E-02
B 31.3 5.80E-02 5.65E-02 5.55E-02
C 33.6 5.80E-02 6.20E-02 6.00E-02
D 37.7 4.65E-02 4.50E-02 4.70E-02
E 42.4 1.80E-02 1.65E-02 1.75E-02
F 45.7 5.00E-04 5.00E-04
G 48.3 5.00E-04 5.00E-04
H 50.0 7.40E-17 0.00E+00

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:09:23.


Design Data

13.581

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.