Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.137

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0335	0.036	0.0355
	B	25.00	0.03	0.027	0.0275
	C	8.33	0.0195	0.0205	0.0195
	D	2.78	0.0125	0.0115	0.011
	E	0.93	0.006	0.0045	0.0045
	F	0.31		0.002	0.002
	G	0.10	0.001	0.001	0.001
	H	0.03	0.0005	0.0005

Yield (mg/mL): 0.1007     Dilution Factor:  100×

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Protein purified on 06/03/2025 and assayed on 27/03/2027.
Data uploaded by nickyw96 and last updated on 2025-04-17 13:25:31.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.0005	0.0005
	B	48.3	0.0005	0.0005	0.0005
	C	45.7	0.0005		0.0005
	D	42.4	0.002	0.002	0.002
	E	37.7	0.0045	0.004	0.005
	F	33.6	0.005	0.0055	0.006
	G	31.3	0.005	0.006	0.005
	H	30.0			0.005

Download these data

Protein purified on 06/03/2025 and assayed on 03/04/2025.
Data uploaded by nickyw96 and last updated on 2025-04-17 14:19:30.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.