D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W407D

Expression Data

Yield (mg/mL): 0.146

Kinetic Assay Data

**slope of W407D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0015	0.0015	0.0015
B	25.00		0.0015	0.0015
C	8.33	0.001	0.001	0.001
D	2.78	0.001	0.001
E	0.93	0.0005	0.0005	0.0005
F	0.31	0.0005	0.0005	0.0005
G	0.10		0.0005	0.0005
H	0.03		1.48E-16	0

Yield (mg/mL): 0.146 Dilution Factor: 10×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by MDIndalsingh and last updated on 2025-04-10 11:36:21.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	7.59E-01	8.06E-01	7.92E-01
C	8.33	5.94E-01	5.93E-01	5.62E-01
D	2.78	3.46E-01	3.59E-01	2.99E-01
E	0.93	1.43E-01	1.45E-01	1.52E-01
F	0.31	5.15E-02	5.25E-02	5.05E-02
G	0.10	1.95E-02	1.95E-02	1.95E-02
H	0.03		5.00E-04	5.00E-04

Yield (mg/mL): 7.764 Dilution Factor: 100×

Protein purified on 3/27/25 and assayed on 4/3/25.
Data uploaded by TDessoffy and last updated on 2025-04-10 11:42:17.

Design Data

Rosetta Score for W407D: -0.076

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.