D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V320Q

Expression Data

Yield (mg/mL): 2.164

Kinetic Assay Data

**slope of V320Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.130600	0.1267	0.1273
B	25.00	0.1492	0.1143	0.1381
C	8.33	0.1035	0.0990	0.0977
D	2.78	0.0572	0.0533	0.0538
E	0.93	0.01970	0.01845	0.01640
F	0.31	0.00785	0.00785	0.00830
G	0.10	0.003000	0.002250	0.002200
H	0.03	0.000200	0.000150	0.000300

Yield (mg/mL): 2.164 Dilution Factor: 100×

Protein purified on 3/3/2025 and assayed on 3/5/2025.
Data uploaded by akalsbury and last updated on 2025-03-11 15:38:25.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.236500	0.2314	0.2359
B	25.00	0.2105	0.1953	0.2035
C	8.33	0.1574	0.1551	0.1521
D	2.78	0.1280	0.1010	0.0841
E	0.93	0.04245	0.04575	0.04070
F	0.31	0.02300	0.01830	0.01585
G	0.10		0.006250	0.005900
H	0.03	0.000150	0.000250	0.000200

Yield (mg/mL): 2.334 Dilution Factor: 100×

Protein purified on 3/3/2025 and assayed on 3/5/2025.
Data uploaded by akalsbury and last updated on 2025-03-11 15:22:18.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.