D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F332H

Expression Data

Yield (mg/mL): 0.57

Kinetic Assay Data

**slope of F332H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.019300	0.0219	0.0197
B	25.00	0.0180	0.0244	0.0215
C	8.33	0.0137	0.0140	0.0163
D	2.78	0.0087	0.0087	0.0084
E	0.93	0.00360	0.00360	0.00325
F	0.31	0.00140	0.00170	0.00160
G	0.10	0.000700	0.000900	0.000900
H	0.03	0.000150	0.000150

Yield (mg/mL): 0.57 Dilution Factor: 100×

Protein purified on 3/3/2025 and assayed on 3/5/2025.
Data uploaded by akalsbury and last updated on 2025-03-11 15:31:08.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.236500	0.2314	0.2359
B	25.00	0.2105	0.1953	0.2035
C	8.33	0.1574	0.1551	0.1521
D	2.78	0.1280	0.1010	0.0841
E	0.93	0.04245	0.04575	0.04070
F	0.31	0.02300	0.01830	0.01585
G	0.10		0.006250	0.005900
H	0.03	0.000150	0.000250	0.000200

Yield (mg/mL): 2.334 Dilution Factor: 100×

Protein purified on 3/3/2025 and assayed on 3/5/2025.
Data uploaded by akalsbury and last updated on 2025-03-11 15:22:18.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.