D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F332H

Expression Data

Yield (mg/mL): 0.45

Kinetic Assay Data

**slope of F332H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.009900	0.0107	0.0105
B	25.00	0.0097	0.0095	0.0101
C	8.33	0.0075	0.0073	0.0068
D	2.78	0.0037	0.0036
E	0.93	0.00170	0.00155
F	0.31	0.00085	0.00070	0.00060
G	0.10	0.000350	0.000400	0.000350
H	0.03	0.000200	0.000200

Yield (mg/mL): 0.45 Dilution Factor: 100×

Protein purified on 3/3/2025 and assayed on 3/5/2025.
Data uploaded by akalsbury and last updated on 2025-03-11 15:14:43.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.236500	0.2314	0.2359
B	25.00	0.2105	0.1953	0.2035
C	8.33	0.1574	0.1551	0.1521
D	2.78	0.1280	0.1010	0.0841
E	0.93	0.04245	0.04575	0.04070
F	0.31	0.02300	0.01830	0.01585
G	0.10		0.006250	0.005900
H	0.03	0.000150	0.000250	0.000200

Yield (mg/mL): 2.334 Dilution Factor: 100×

Protein purified on 3/3/2025 and assayed on 3/5/2025.
Data uploaded by akalsbury and last updated on 2025-03-11 15:26:48.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.