D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178Q

Expression Data

Yield (mg/mL): 1.394

Kinetic Assay Data

**slope of H178Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2849	0.2993	0.3614
B	25.00	0.2565	0.2667	0.2647
C	8.33	0.1874	0.2082
D	2.78	0.0857	0.0966	0.1057
E	0.93	0.03500	0.03900
F	0.31		0.01270	0.01885
G	0.10		0.005350	0.006301
H	0.03	0.000250	0	0.000350

Yield (mg/mL): 1.394 Dilution Factor: 100×

Protein purified on 2/24/2025 and assayed on 2/28/2025.
Data uploaded by lfarinsky and last updated on 2025-03-06 17:29:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2547	0.2471	0.2920
B	25.00	0.2188	0.2281	0.2135
C	8.33	0.1790	0.1543	0.1495
D	2.78	0.0826	0.0842	0.0903
E	0.93	0.03765	0.03415	0.03825
F	0.31	0.01345	0.01090	0.01350
G	0.10	0.003850	0.003550	0.004550
H	0.03	0	0.000150	0.000450

Yield (mg/mL): 3.382 Dilution Factor: 100×

Protein purified on 2/24/2025 and assayed on 2/28/2025.
Data uploaded by lfarinsky and last updated on 2025-03-06 17:27:55.

Design Data

Rosetta Score for H178Q: 2.583

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.