D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R135I

Expression Data

Yield (mg/mL): 0.167

Kinetic Assay Data

**slope of R135I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.39E-01	1.39E-01	1.42E-01
B	25.00	1.17E-01	1.10E-01	1.11E-01
C	8.33	9.25E-02	9.60E-02	1.02E-01
D	2.78	5.00E-02	5.25E-02	5.10E-02
E	0.93	2.45E-02	2.40E-02	2.25E-02
F	0.31	1.10E-02	1.00E-02	1.00E-02
G	0.10	4.00E-03	4.00E-03	4.00E-03
H	0.03		0.00E+00	0.00E+00

Yield (mg/mL): 0.167 Dilution Factor: 10×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by jemilalvarez and last updated on 2025-04-10 11:50:26.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33	6.97E-01	7.17E-01	7.40E-01
D	2.78	5.82E-01	4.58E-01	4.78E-01
E	0.93	2.52E-01	1.90E-01	1.95E-01
F	0.31		7.35E-02	7.95E-02
G	0.10	4.45E-02	2.85E-02	3.35E-02
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 8.308 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by jemilalvarez and last updated on 2025-04-10 11:59:21.

Design Data

Rosetta Score for R135I: 6.102

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.