D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R135S

Expression Data

Yield (mg/mL): 0.047

Kinetic Assay Data

**slope of R135S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.85E-02	1.90E-02	2.00E-02
B	25.00	1.75E-02	1.75E-02	1.70E-02
C	8.33	1.30E-02	1.25E-02	1.20E-02
D	2.78	7.00E-03	7.50E-03	7.00E-03
E	0.93	3.50E-03	3.50E-03	3.00E-03
F	0.31	1.50E-03	1.50E-03	1.50E-03
G	0.10	1.00E-03		1.00E-03
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 0.047 Dilution Factor: 10×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by AJCROSSMAN and last updated on 2025-04-10 11:50:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33	6.97E-01	7.17E-01	7.40E-01
D	2.78	5.82E-01	4.58E-01	4.78E-01
E	0.93	2.52E-01	1.90E-01	1.95E-01
F	0.31		7.35E-02	7.95E-02
G	0.10	4.45E-02	2.85E-02	3.35E-02
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 8.308 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by jemilalvarez and last updated on 2025-04-10 11:59:21.

Design Data

Rosetta Score for R135S: 3.485

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.