D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K413H

Expression Data

Yield (mg/mL): 5.114

Kinetic Assay Data

**slope of K413H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33
D	2.78	8.62E-01	7.88E-01	9.23E-01
E	0.93	5.24E-01	4.67E-01	4.90E-01
F	0.31	1.77E-01	1.57E-01	1.51E-01
G	0.10	5.00E-02	5.05E-02	5.20E-02
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 5.114 Dilution Factor: 100×

Protein purified on 3/28/2025 and assayed on 4/3/2025.
Data uploaded by RayvenDenver01 and last updated on 2025-04-10 11:38:07.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33
D	2.78	6.49E-01	5.04E-01
E	0.93	5.09E-01	5.77E-01	5.95E-01
F	0.31	1.79E-01	1.82E-01	1.87E-01
G	0.10	5.75E-02	5.65E-02	6.15E-02
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 6.117 Dilution Factor: 10×

Protein purified on 3/28/25 and assayed on 4/3/25.
Data uploaded by Mxdecourcey and last updated on 2025-04-10 11:38:43.

Design Data

Rosetta Score for K413H: 2

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.