D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D279Q

Expression Data

Yield (mg/mL): 0.339

Kinetic Assay Data

**slope of D279Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.00E-03	7.50E-03	7.00E-03
B	25.00	6.00E-03	6.00E-03
C	8.33	4.50E-03	5.00E-03	5.00E-03
D	2.78	2.50E-03	2.50E-03	2.50E-03
E	0.93		1.00E-03	1.00E-03
F	0.31	5.00E-04		5.00E-04
G	0.10	5.00E-04	5.00E-04	5.00E-04
H	0.03	5.00E-04	5.00E-04

Yield (mg/mL): 0.339 Dilution Factor: 10×

Protein purified on 3/28/25 and assayed on 4/3/25.
Data uploaded by Mxdecourcey and last updated on 2025-04-10 11:19:20.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33
D	2.78	6.49E-01	5.04E-01
E	0.93	5.09E-01	5.77E-01	5.95E-01
F	0.31	1.79E-01	1.82E-01	1.87E-01
G	0.10	5.75E-02	5.65E-02	6.15E-02
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 6.117 Dilution Factor: 10×

Protein purified on 3/28/25 and assayed on 4/3/25.
Data uploaded by Mxdecourcey and last updated on 2025-04-10 11:38:43.

Design Data

Rosetta Score for D279Q: 31.697

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.