D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D279T

Expression Data

Yield (mg/mL): 0.504

Kinetic Assay Data

**slope of D279T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.00E-02	7.30E-02	7.50E-02
B	25.00	5.45E-02	5.50E-02	5.55E-02
C	8.33	3.95E-02	3.95E-02
D	2.78	2.60E-02	2.65E-02	2.70E-02
E	0.93	1.05E-02	1.00E-02	1.10E-02
F	0.31	4.00E-03	4.00E-03	4.00E-03
G	0.10	1.50E-03	1.50E-03	1.50E-03
H	0.03		0.00E+00	0.00E+00

Yield (mg/mL): 0.504 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by victoriar and last updated on 2025-04-10 11:41:19.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33		8.68E-01	8.79E-01
D	2.78	5.63E-01	4.93E-01	5.10E-01
E	0.93	2.47E-01	2.13E-01	2.24E-01
F	0.31	8.35E-02	8.25E-02	7.70E-02
G	0.10	3.15E-02	3.30E-02	2.65E-02
H	0.03	5.00E-04		5.00E-04

Yield (mg/mL): 10.365 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by victoriar and last updated on 2025-04-10 11:42:58.

Design Data

Rosetta Score for D279T: 10.3

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.