D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R135L

Expression Data

Yield (mg/mL): 0.365

Kinetic Assay Data

**slope of R135L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.25E-02	3.10E-02	2.95E-02
B	25.00	2.50E-02	2.45E-02	2.45E-02
C	8.33	1.95E-02	1.85E-02	1.70E-02
D	2.78	1.20E-02	1.25E-02	1.20E-02
E	0.93	5.00E-03	5.00E-03	4.50E-03
F	0.31	2.00E-03		2.00E-03
G	0.10		1.00E-03	1.00E-03
H	0.03		1.48E-16	5.00E-04

Yield (mg/mL): 0.365 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by KayleighColeman and last updated on 2025-04-10 11:35:14.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33		8.68E-01	8.79E-01
D	2.78	5.63E-01	4.93E-01	5.10E-01
E	0.93	2.47E-01	2.13E-01	2.24E-01
F	0.31	8.35E-02	8.25E-02	7.70E-02
G	0.10	3.15E-02	3.30E-02	2.65E-02
H	0.03	5.00E-04		5.00E-04

Yield (mg/mL): 10.365 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by victoriar and last updated on 2025-04-10 11:42:58.

Design Data

Rosetta Score for R135L: 5.069

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.