D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120D

Expression Data

Yield (mg/mL): 0.746

Kinetic Assay Data

**slope of W120D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.05E-03	2.45E-03
B	25.00		1.05E-03	9.50E-04
C	8.33	8.00E-04	8.50E-04
D	2.78	4.00E-04	5.00E-04
E	0.93		3.00E-04	3.50E-04
F	0.31		3.50E-04
G	0.10	2.00E-04	2.00E-04
H	0.03

Yield (mg/mL): 0.746 Dilution Factor: 10×

Protein purified on 2/24/25 and assayed on 2/26/25.
Data uploaded by Mixin and last updated on 2025-03-05 16:04:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.02E-01	2.08E-01	2.36E-01
B	25.00	2.42E-01	2.18E-01	2.12E-01
C	8.33	1.16E-01	9.62E-02	1.19E-01
D	2.78	3.98E-02	4.65E-02	5.44E-02
E	0.93	1.32E-02	1.60E-02	2.37E-02
F	0.31	4.55E-03	5.35E-03	6.95E-03
G	0.10	1.30E-03	1.90E-03	2.30E-03
H	0.03	3.00E-04	3.50E-04

Yield (mg/mL): 3.212 Dilution Factor: 100×

Protein purified on 2/24/25 and assayed on 2/26/25.
Data uploaded by Mixin and last updated on 2025-02-28 15:20:46.

Design Data

Rosetta Score for W120D: 5.743

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.