D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E183T

Expression Data

Yield (mg/mL): 3.573

Kinetic Assay Data

**slope of E183T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.74E-01	2.80E-01	2.72E-01
B	25.00	2.10E-01	1.98E-01	2.11E-01
C	8.33	1.96E-01	1.68E-01	1.70E-01
D	2.78	1.27E-01	1.40E-01	1.48E-01
E	0.93	7.10E-02	7.96E-02	7.12E-02
F	0.31		4.46E-02	4.19E-02
G	0.10	1.01E-02	1.89E-02	1.49E-02
H	0.03	2.00E-04	2.50E-04	1.50E-04

Yield (mg/mL): 3.573 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by ttylam and last updated on 2025-03-03 17:15:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.87E-01	3.01E-01	3.05E-01
B	25.00	2.49E-01	2.54E-01	2.54E-01
C	8.33	2.48E-01	2.40E-01	2.28E-01
D	2.78	1.91E-01	1.92E-01	1.74E-01
E	0.93	1.37E-01	1.27E-01	1.07E-01
F	0.31	7.57E-02	6.66E-02	4.50E-02
G	0.10	2.49E-02	2.67E-02
H	0.03	2.00E-04	1.50E-04	2.00E-04

Yield (mg/mL): 2.913 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by ttylam and last updated on 2025-03-03 17:02:03.

Design Data

Rosetta Score for E183T: 0.674

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.