D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L171H

Expression Data

Yield (mg/mL): 4.515

Kinetic Assay Data

**slope of L171H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.01E-01	2.93E-01	2.58E-01
B	25.00	2.28E-01	2.44E-01	2.12E-01
C	8.33	2.03E-01	2.15E-01	2.09E-01
D	2.78	1.39E-01	1.25E-01	1.60E-01
E	0.93	6.91E-02	5.73E-02	9.21E-02
F	0.31	3.77E-02	2.41E-02	5.06E-02
G	0.10	1.03E-02	9.20E-03
H	0.03	3.00E-04	2.50E-04

Yield (mg/mL): 4.515 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by ttylam and last updated on 2025-03-03 16:15:37.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.87E-01	3.01E-01	3.05E-01
B	25.00	2.49E-01	2.54E-01	2.54E-01
C	8.33	2.48E-01	2.40E-01	2.28E-01
D	2.78	1.91E-01	1.92E-01	1.74E-01
E	0.93	1.37E-01	1.27E-01	1.07E-01
F	0.31	7.57E-02	6.66E-02	4.50E-02
G	0.10	2.49E-02	2.67E-02
H	0.03	2.00E-04	1.50E-04	2.00E-04

Yield (mg/mL): 2.913 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by ttylam and last updated on 2025-03-03 17:02:03.

Design Data

Rosetta Score for L171H: -2.848

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.