D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I299R

Expression Data

Yield (mg/mL): 2.668

Kinetic Assay Data

**slope of I299R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2916	0.2807	0.2960
B	25.00	0.2288	0.2197	0.2343
C	8.33	0.1651	0.1741	0.1743
D	2.78	0.0928	0.0943	0.1099
E	0.93	0.05030	0.04185	0.04240
F	0.31	0.01725	0.01755	0.01535
G	0.10	0.006501	0.005800	0.005200
H	0.03	0.000650	0.000750	0.000650

Yield (mg/mL): 2.668 Dilution Factor: 100×

Protein purified on 2/3/2025 and assayed on 2/4/2025.
Data uploaded by LoganPetit and last updated on 2025-02-24 16:17:59.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4148	0.4251	0.3884
B	25.00	0.3576	0.3671	0.3456
C	8.33	0.2606	0.2717	0.2890
D	2.78	0.1495	0.1926	0.2211
E	0.93	0.07400	0.08336	0.08720
F	0.31	0.03130	0.03215	0.03395
G	0.10	0.010600	0.012100	0.014100
H	0.03	0.000900	0.000800

Yield (mg/mL): 4.6 Dilution Factor: 100×

Protein purified on 2/3/2025 and assayed on 2/4/2025.
Data uploaded by LoganPetit and last updated on 2025-02-24 15:54:07.

Design Data

Rosetta Score for I299R: 3.443

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.