D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L272F

Expression Data

Yield (mg/mL): 1.013

Kinetic Assay Data

**slope of L272F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.75E-03		3.25E-03
B	25.00	2.35E-03	2.00E-03	2.05E-03
C	8.33	1.70E-03	1.60E-03	1.30E-03
D	2.78	1.10E-03	1.00E-03	9.00E-04
E	0.93	5.50E-04	5.00E-04
F	0.31	3.50E-04		4.00E-04
G	0.10		2.00E-04	2.00E-04
H	0.03

Yield (mg/mL): 1.013 Dilution Factor: 10×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-20 15:03:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for L272F: 10.716

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.