D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E250D

Expression Data

Yield (mg/mL): 4.259

Kinetic Assay Data

**slope of E250D A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	4.19E-01	4.08E-01
B	25.00	3.48E-01	3.45E-01
C	8.33	2.73E-01	2.98E-01
D	2.78	2.31E-01	2.08E-01
E	0.93	9.68E-02	1.08E-01
F	0.31	3.70E-02	4.00E-02
G	0.10	1.52E-02	1.78E-02
H	0.03	2.50E-04

Yield (mg/mL): 4.259 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-20 14:01:36.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for E250D: 4.597

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.