D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A226P

Expression Data

Yield (mg/mL): 4.507

Kinetic Assay Data

**slope of A226P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		4.28E-01	4.33E-01
B	25.00	3.97E-01	3.56E-01	3.81E-01
C	8.33	3.34E-01	2.85E-01	2.93E-01
D	2.78	2.43E-01	1.82E-01	2.08E-01
E	0.93	1.15E-01	9.84E-02	9.13E-02
F	0.31	4.88E-02	3.42E-02	3.99E-02
G	0.10		1.04E-02	1.15E-02
H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 4.507 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 18:31:08.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for A226P: 3.025

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.