D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A179S

Expression Data

Yield (mg/mL): 1.575

Kinetic Assay Data

**slope of A179S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.59E-02	5.66E-02	5.84E-02
B	25.00	5.44E-02	5.24E-02	5.23E-02
C	8.33	4.11E-02	3.92E-02	4.84E-02
D	2.78		3.22E-02	3.29E-02
E	0.93	1.66E-02	1.68E-02	1.39E-02
F	0.31	7.35E-03	6.30E-03	5.40E-03
G	0.10	2.10E-03	2.00E-03	2.00E-03
H	0.03		0	0

Yield (mg/mL): 1.575 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 18:23:21.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for A179S: 6.788

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.