D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D121Q

Expression Data

Yield (mg/mL): 1.361

Kinetic Assay Data

**slope of D121Q A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	7.25E-03	7.45E-03
B	25.00	4.10E-03	4.15E-03
C	8.33	3.65E-03	2.95E-03
D	2.78	1.80E-03	1.80E-03
E	0.93	7.50E-04	6.50E-04
F	0.31	4.00E-04
G	0.10		2.00E-04
H	0.03		0

Yield (mg/mL): 1.361 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 17:34:24.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for D121Q: 8.905

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.