D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N184D

Expression Data

Yield (mg/mL): 1.076

Kinetic Assay Data

**slope of N184D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		7.83E-02	7.76E-02
C	8.33	6.53E-02	5.93E-02	6.12E-02
D	2.78	4.31E-02	4.13E-02	3.88E-02
E	0.93	1.99E-02	2.42E-02	2.67E-02
F	0.31	8.95E-03	8.75E-03	8.85E-03
G	0.10	3.45E-03	3.15E-03	3.25E-03
H	0.03		3.50E-04	3.50E-04

Yield (mg/mL): 1.076 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 16:33:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		8.12E-02	8.47E-02
C	8.33	7.32E-02	6.56E-02	6.36E-02
D	2.78		4.01E-02	3.71E-02
E	0.93	2.84E-02	2.01E-02	2.60E-02
F	0.31	1.21E-02	1.20E-02	8.75E-03
G	0.10	3.75E-03	3.70E-03	3.95E-03
H	0.03	0	0

Yield (mg/mL): 1.446 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 16:28:40.

Design Data

Rosetta Score for N184D: -0.193

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.