D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S14A

Expression Data

Yield (mg/mL): 1.692

Kinetic Assay Data

**slope of S14A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.00E-01	9.62E-02	8.56E-02
B	25.00	8.45E-02	7.29E-02	6.89E-02
C	8.33	6.50E-02	5.43E-02	5.13E-02
D	2.78	5.41E-02	4.25E-02	4.54E-02
E	0.93	2.69E-02	2.40E-02	2.51E-02
F	0.31	1.22E-02	1.11E-02	1.16E-02
G	0.10	4.55E-03	4.30E-03	4.65E-03
H	0.03		0	3.00E-04

Yield (mg/mL): 1.692 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 15:23:39.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		8.79E-02	9.15E-02
B	25.00	8.64E-02	7.16E-02	7.52E-02
C	8.33	7.54E-02	7.23E-02	6.81E-02
D	2.78	5.46E-02	5.77E-02	4.57E-02
E	0.93	2.33E-02	3.20E-02	1.90E-02
F	0.31	7.25E-03		7.60E-03
G	0.10	5.85E-03	5.20E-03
H	0.03		1.50E-04	2.50E-04

Yield (mg/mL): 1.432 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 15:12:09.

Design Data

Rosetta Score for S14A: 1.765

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.