D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404D

Expression Data

Yield (mg/mL): 1.69

Kinetic Assay Data

**slope of N404D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2583	0.3249	0.2608
B	25.00	0.2245	0.2062	0.2125
C	8.33	0.1667	0.1588	0.1753
D	2.78	0.1236	0.1038	0.1218
E	0.93	0.05315	0.05420	0.06825
F	0.31	0.02450	0.02940	0.02590
G	0.10	0.010950	0.009750	0.010050
H	0.03	0.003650	0.002800	0.003700

Yield (mg/mL): 1.69 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by amaduros and last updated on 2025-01-13 17:15:43.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2328	0.2381	0.2455
B	25.00
C	8.33	0.1775	0.1712	0.1504
D	2.78	0.1043	0.1030	0.1105
E	0.93	0.04690	0.03935	0.03970
F	0.31	0.02235	0.01880	0.01915
G	0.10	0.008750	0.006250	0.006550
H	0.03	0.002450	0.002550	0.002550

Yield (mg/mL): 1.614 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by amaduros and last updated on 2025-01-15 23:12:33.

Design Data

Rosetta Score for N404D: 5.662

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.