D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y75F

Expression Data

Yield (mg/mL): 3.87

Kinetic Assay Data

**slope of Y75F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.51E-01	2.82E-01
B	25.00	2.44E-01	2.80E-01	1.94E-01
C	8.33	6.42E-02	1.56E-01	1.93E-01
D	2.78	5.90E-02	8.92E-02	1.04E-01
E	0.93		8.12E-02	7.09E-02
F	0.31	4.51E-02	5.29E-02	3.42E-02
G	0.10	1.71E-02	2.00E-02	1.63E-02
H	0.03	7.00E-05	8.00E-05	1.00E-04

Yield (mg/mL): 3.87 Dilution Factor: 100×

Protein purified on 6/2/24 and assayed on 6/5/24.
Data uploaded by kuhumukherjee and last updated on 2025-01-16 11:08:06.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.32E-01	2.45E-01	2.14E-01
B	25.00	1.90E-01	1.69E-01	1.73E-01
C	8.33	1.52E-01	1.27E-01	1.56E-01
D	2.78	1.07E-01	1.17E-01	1.03E-01
E	0.93	8.74E-02	4.90E-02	7.09E-02
F	0.31	5.09E-02	2.46E-02	3.39E-02
G	0.10	1.34E-02	1.04E-02	1.49E-02
H	0.03	4.00E-05	1.10E-04	8.00E-05

Yield (mg/mL): 3.81 Dilution Factor: 100×

Protein purified on 6/2/24 and assayed on 6/5/24.
Data uploaded by kuhumukherjee and last updated on 2025-01-15 23:07:21.

Design Data

Rosetta Score for Y75F: 2.028

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.