D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F397T

Expression Data

Yield (mg/mL): 0.4752

Kinetic Assay Data

**slope of F397T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0477	0.0495	0.05025
B	25.00	0.0459	0.0369	0.0414
C	8.33	0.0411	0.0423	0.0354
D	2.78	0.03255	0.03165	0.03465
E	0.93	0.01575	0.02025	0.0192
F	0.31	0.00855	0.009903	0.0093
G	0.10	0.00435	0.00465	0.00435
H	0.03	0	0

Yield (mg/mL): 0.4752 Dilution Factor: 10×

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 23:05:20.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1617	0.1625	0.158
B	25.00	0.1239	0.1195	0.109
C	8.33	0.1262	0.1027	0.1074
D	2.78	0.09285	0.0939	0.1031
E	0.93	0.06105	0.04965	0.0525
F	0.31	0.02865	0.0297	0.02895
G	0.10	0.01335	0.0126	0.0117
H	0.03	0.0006	0.00075

Yield (mg/mL): 3.747 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 22:43:22.

Thermostability Assay Data

**slope of F397T A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	0.0192	0.0189	0.01815
B	31.3	0.0165	0.01455	0.0135
C	33.6	0.01125	0.01065	0.0108
D	37.7	0.006	0.00525	0.00495
E	42.4	0.0009	0.0009
F	45.7	0.0006	0.0006	0.00075
G	48.3	0.0006	0.00075	0.0006
H	50.0		0.0006	0.0006

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 23:08:23.

Reference Data for WT

**slope of F397T A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	0.1015	0.103	0.09915
B	31.3	0.09825	0.1021	0.0963
C	33.6	0.07785	0.08925	0.0915
D	37.7	0.09075	0.0897	0.087
E	42.4	0.01305	0.0123	0.012
F	45.7	0.00075	0.00075	0.0009
G	48.3	0.00075	0.0006	0.0006002
H	50.0	0		0

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 22:46:32.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.