Raw Data for Wild-Type BglB

Expression Data

No expression

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.90E-01	4.56E-01	3.00E-02
	B	25.00	9.00E-02	3.10E-01	9.70E-01
	C	8.33	2.10E-01	2.50E-01
	D	2.78	1.90E-01	2.50E-01	3.00E-02
	E	0.93	9.00E-02	1.40E-01	3.00E-02
	F	0.31	1.49E-01		5.00E-02
	G	0.10		3.00E-02	5.00E-02
	H	0.03	2.92E+00	1.09E+00	1.70E-01

Yield (mg/mL): 0.364     Dilution Factor:  10×

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Protein purified on 10/11/24 and assayed on 11/5/24.
Data uploaded by rbreitlow and last updated on 2024-12-11 13:41:27.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.70E-01		1.70E-01
	B	48.3	2.40E-01	2.30E-01	2.50E-01
	C	45.7	1.80E-01	2.78E-01
	D	42.4
	E	37.7	1.80E-01		4.00E-02
	F	33.6		9.00E-02	6.00E-02
	G	31.3		5.00E-02	7.00E-02
	H	30.0	6.00E-02	2.00E-02

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Protein purified on 11/4/24 and assayed on 11/19/24.
Data uploaded by rbreitlow and last updated on 2024-12-11 13:42:40.

Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.