Raw Data for BglB Variant W409A

Expression Data

SDS-PAGE
 0.67

Kinetic Assay Data

slope of W409A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0485 0.0495 0.0515
B 25.00 0.0335 0.03 0.029
C 8.33 0.028 0.025 0.019
D 2.78 0.014 0.0125 0.015
E 0.93 0.009 0.007 0.0065
F 0.31 0.003 0.003 0.0025
G 0.10 0.0015 0.0015
H 0.03 0.0005
 0.67      100×

Protein purified on 10/24/2024 and assayed on 10/31/2024.
Data uploaded by Erica-Hooshi and last updated on 2024-12-05 14:52:38.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of W409A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.401E-17
B 48.3 0.0005 0.0005 0.0005
C 45.7 0.001 0.001
D 42.4 0.0105 0.0095 0.0105
E 37.7 0.0195 0.0205 0.019
F 33.6 0.025 0.0245 0.024
G 31.3 0.0245 0.0225 0.024
H 30.0 0.0225 0.027 0.0265

Protein purified on 10/24/24 and assayed on 11/7/24.
Data uploaded by Erica-Hooshi and last updated on 2024-12-05 15:04:53.


Reference Data for WT

slope of W409A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

4.164

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.