D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409Y

Yield (mg/mL): 1.445

**slope of W409Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.23E-01	2.21E-01	2.22E-01
B	25.00	1.86E-01	1.86E-01	1.81E-01
C	8.33	1.51E-01	1.51E-01	1.48E-01
D	2.78	1.03E-01	1.06E-01	1.01E-01
E	0.93	5.35E-02	5.50E-02	5.10E-02
F	0.31		2.35E-02	2.30E-02
G	0.10	8.00E-03	8.50E-03	7.50E-03
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 1.445 Dilution Factor: 100×

Protein purified on 10/24/24 and assayed on 11/6/24.
Data uploaded by raywipfli and last updated on 2024-12-05 14:53:19.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.88E-01	1.82E-01	1.79E-01
B	25.00	1.49E-01	1.52E-01	1.47E-01
C	8.33	1.35E-01	1.37E-01	1.39E-01
D	2.78	6.51E-02	6.22E-02	6.55E-02
E	0.93	4.34E-02	4.43E-02	4.18E-02
F	0.31	2.02E-02	1.64E-02	1.69E-02
G	0.10	9.45E-03	1.03E-02	7.65E-03
H	0.03	3.40E-03	3.45E-03	2.45E-03

Yield (mg/mL): 2.377 Dilution Factor: 100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.

**slope of W409Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	5.00E-04	5.00E-04
B	48.3	1.48E-16	1.48E-16
C	45.7	1.00E-03	1.00E-03
D	42.4	6.65E-02	8.45E-02	6.85E-02
E	37.7	1.73E-01	2.26E-01
F	33.6	1.71E-01	2.27E-01
G	31.3	2.27E-01	2.56E-01	2.51E-01
H	30.0	2.43E-01	2.52E-01	2.32E-01

Protein purified on 10/24/24 and assayed on 11/14/24.
Data uploaded by raywipfli and last updated on 2024-12-05 14:59:39.

**slope of W409Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	7.00E-04
B	48.3	4.00E-04	5.00E-04	6.00E-04
C	45.7	6.00E-04	9.00E-04	7.00E-04
D	42.4	4.12E-02	3.96E-02	4.71E-02
E	37.7	2.14E-01	1.91E-01	2.10E-01
F	33.6	2.47E-01	2.35E-01	2.36E-01
G	31.3	2.18E-01	2.14E-01	2.05E-01
H	30.0	2.62E-01	2.56E-01	2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.

Rosetta Score for W409Y: 0.391

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.