D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W125F

Yield (mg/mL): 1.6

**slope of W125F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.17E-01	2.22E-01	2.28E-01
B	25.00	1.63E-01	1.88E-01	1.82E-01
C	8.33	1.36E-01	1.44E-01	1.43E-01
D	2.78	8.20E-02	9.50E-02	9.00E-02
E	0.93	3.75E-02	4.75E-02
F	0.31	1.35E-02	1.80E-02	2.00E-03
G	0.10		1.50E-03	5.00E-04
H	0.03	1.00E-03	1.00E-03	1.00E-03

Yield (mg/mL): 1.6 Dilution Factor: 10×

Protein purified on 10/24/24 and assayed on 11/7/24.
Data uploaded by Joseph123 and last updated on 2024-12-05 14:25:31.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.88E-01	1.82E-01	1.79E-01
B	25.00	1.49E-01	1.52E-01	1.47E-01
C	8.33	1.35E-01	1.37E-01	1.39E-01
D	2.78	6.51E-02	6.22E-02	6.55E-02
E	0.93	4.34E-02	4.43E-02	4.18E-02
F	0.31	2.02E-02	1.64E-02	1.69E-02
G	0.10	9.45E-03	1.03E-02	7.65E-03
H	0.03	3.40E-03	3.45E-03	2.45E-03

Yield (mg/mL): 2.377 Dilution Factor: 100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.

**slope of W125F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.00E+00		0.00E+00
B	48.3	5.00E-04		5.00E-04
C	45.7	0.00E+00	0	0.00E+00
D	42.4	2.50E-03	2.50E-03	2.00E-03
E	37.7	8.30E-02	1.07E-01	9.45E-02
F	33.6	1.59E-01		1.58E-01
G	31.3	1.78E-01	2.49E-01	1.52E-01
H	30.0	1.72E-01	1.97E-01	1.77E-01

Protein purified on 10/24/24 and assayed on 11/14/24.
Data uploaded by Joseph123 and last updated on 2024-12-05 14:45:05.

Rosetta Score for W125F: 6.327

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.