Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.006

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.37E-02	2.33E-02	2.15E-02
	B	25.00	1.05E-02	1.01E-02	8.50E-03
	C	8.33	3.35E-03	3.10E-03	3.30E-03
	D	2.78	1.40E-03	1.50E-03	1.65E-03
	E	0.93	8.50E-04	6.50E-04	6.00E-04
	F	0.31	4.50E-04	3.50E-04	5.50E-04
	G	0.10	2.50E-04	3.00E-04	3.50E-04
	H	0.03	2.00E-04	2.00E-04	2.00E-04

Yield (mg/mL): 1.006     Dilution Factor:  10×

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Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by hananalhamami and last updated on 2024-12-05 12:43:58.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.50E-04		2.50E-04
	B	48.3	2.50E-04		2.00E-04
	C	45.7	2.50E-04	2.00E-04
	D	42.4	9.00E-04	9.00E-04	1.05E-03
	E	37.7	3.50E-03	3.30E-03	3.70E-03
	F	33.6	3.80E-03	3.70E-03	3.85E-03
	G	31.3		3.80E-03	3.90E-03
	H	30.0	4.15E-03	4.25E-03	4.30E-03

Download these data

Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by hananalhamami and last updated on 2024-12-05 12:48:37.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.