Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.06667

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.72E-02	2.79E-02	2.67E-02
	B	25.00	1.17E-02	1.16E-02	1.01E-02
	C	8.33	4.95E-03	4.65E-03	3.95E-03
	D	2.78	1.70E-03	1.60E-03	1.45E-03
	E	0.93	8.50E-04	7.00E-04	6.00E-04
	F	0.31	3.50E-04	4.00E-04	3.00E-04
	G	0.10	7.00E-04	1.50E-04
	H	0.03	3.00E-04	0.00E+00	1.00E-04

Yield (mg/mL): 1.06667     Dilution Factor:  100×

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Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by Ivonne and last updated on 2024-12-05 12:40:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.00E-04	2.00E-04
	B	48.3	1.05E-03	3.50E-04	0.00E+00
	C	45.7		0.00E+00	0.00E+00
	D	42.4	8.50E-04	1.60E-03	1.20E-03
	E	37.7	4.55E-03	4.50E-03	4.35E-03
	F	33.6	3.90E-03	3.40E-03	3.55E-03
	G	31.3	5.00E-03	4.60E-03	4.30E-03
	H	30.0	5.40E-03	6.15E-03	5.10E-03

Download these data

Protein purified on 10/31/2024 and assayed on 11/9/2024.
Data uploaded by Ivonne and last updated on 2024-12-05 12:31:29.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.