Raw Data for BglB Variant F289C

Expression Data

Yield (mg/mL): 0.1043

Thermostability Assay Data

slope of F289C A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.50E-04	2.00E-04	3.50E-04
	B	48.3	2.00E-04	2.00E-04	2.00E-04
	C	45.7	2.50E-04	2.50E-04	2.00E-04
	D	42.4		2.00E-04	2.00E-04
	E	37.7	2.50E-04	2.00E-04	2.00E-04
	F	33.6	2.50E-04	3.00E-04	1.50E-04
	G	31.3	2.00E-04	2.50E-04	2.00E-04
	H	30.0	3.00E-04		3.00E-04

Download these data

Protein purified on 10/31/2024 and assayed on 11/9/2024.
Data uploaded by Celeste and last updated on 2024-12-05 12:31:21.

Reference Data for WT

slope of F289C A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.00E-04	2.00E-04
	B	48.3	1.05E-03	3.50E-04	0.00E+00
	C	45.7		0.00E+00	0.00E+00
	D	42.4	8.50E-04	1.60E-03	1.20E-03
	E	37.7	4.55E-03	4.50E-03	4.35E-03
	F	33.6	3.90E-03	3.40E-03	3.55E-03
	G	31.3	5.00E-03	4.60E-03	4.30E-03
	H	30.0	5.40E-03	6.15E-03	5.10E-03

Download these data

Protein purified on 10/31/2024 and assayed on 11/9/2024.
Data uploaded by Ivonne and last updated on 2024-12-05 12:31:29.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.