Raw Data for BglB Variant E154I

Expression Data

SDS-PAGE
 0.668

Kinetic Assay Data

slope of E154I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.97E+02 2.00E+02 2.02E+02
B 25.00 1.53E+02 1.57E+02 1.52E+02
C 8.33 1.24E+02 1.33E+02 1.30E+02
D 2.78 8.38E+01 8.38E+01 8.72E+01
E 0.93 4.13E+01 4.38E+01 4.82E+01
F 0.31 1.51E+01 1.62E+01 1.97E+01
G 0.10 6.00E-02 6.00E-02
H 0.03 3.00E-02 5.00E-02 6.00E-02
 0.668      10×

Protein purified on and assayed on .
Data uploaded by connorradtke9 and last updated on 2024-12-12 10:22:41.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 196.518 200.219 201.575
B 25.00 153.305 157.123 152.092
C 8.33 124.35 132.67 130.08
D 2.78 83.78 83.813 87.229
E 0.93 41.339 43.794 48.194
F 0.31 15.089 16.177 19.668
G 0.10 0.06 0.06
H 0.03 0.03 0.05 0.06
 0.327      10×

Protein purified on and assayed on .
Data uploaded by ChaseM and last updated on 2024-12-05 12:04:48.


Thermostability Assay Data

slope of E154I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 8.00E-02 8.00E-02
B 48.3 1.50E-01 5.50E-01 0.00E+00
C 45.7 8.00E-02 2.10E-01 2.70E-01
D 42.4 2.20E+00 1.95E+00
E 37.7 1.87E+01 1.44E+01
F 33.6 4.00E-02 1.05E+01 2.07E+01
G 31.3 1.21E+01 3.00E-02 1.64E+01
H 30.0 1.90E+01 1.57E+01

Protein purified on 11/7/2024 and assayed on 11/21/2024.
Data uploaded by connorradtke9 and last updated on 2024-12-12 10:51:50.


Reference Data for WT

slope of E154I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-02 8.00E-02 1.60E-01
B 48.3 1.50E-01 5.50E-01 0.00E+00
C 45.7 8.00E-02 2.10E-01 2.70E-01
D 42.4 2.20E+00 1.95E+00
E 37.7 1.87E+01 1.44E+01
F 33.6 4.00E-02 1.05E+01 2.07E+01
G 31.3 1.21E+01 3.00E-02 1.64E+01
H 30.0 1.90E+01 1.57E+01

Protein purified on 11/7/24 and assayed on 11/21/24.
Data uploaded by ChaseM and last updated on 2024-12-05 12:27:47.


Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.