Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.449

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	356.368	351.631	393.404
	B	25.00		14.043	14.79
	C	8.33
	D	2.78
	E	0.93
	F	0.31
	G	0.10	7.053	5.824	5.537
	H	0.03	0	0

Yield (mg/mL): 0.449     Dilution Factor:  10×

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Protein purified on 11/7/24 and assayed on 11/14/24.
Data uploaded by KylerThruston and last updated on 2024-12-05 12:06:35.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0
	B	48.3		0.6	0.5
	C	45.7	1.194	1.165	1.34
	D	42.4
	E	37.7	227.13	217.43	226.02
	F	33.6		267.52	316.21
	G	31.3	181.85	234.03	226.79
	H	50.0	231.05		218.11

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Protein purified on 11/7/24 and assayed on 11/21/24.
Data uploaded by KylerThruston and last updated on 2024-12-05 12:16:07.

Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.