Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.286

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.85E+00	3.82E+00	3.64E+00
	B	25.00	3.85E+00	3.69E+00	3.38E+00
	C	8.33	3.51E+00	3.25E+00	2.77E+00
	D	2.78	2.55E+00	2.30E+00	1.79E+00
	E	0.93	1.37E+00	1.04E+00	6.92E-01
	F	0.31	6.51E-01	5.09E-01	3.63E-01
	G	0.10	2.55E-01	2.36E-01
	H	0.03	4.20E-02	4.20E-02	4.60E-02

Yield (mg/mL): 1.286     Dilution Factor:  10×

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Protein purified on 11/7/2024 and assayed on 11/14/2024.
Data uploaded by Autumnbayerl and last updated on 2024-12-05 09:17:55.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.14		1.30E-01
	B	48.3	2.68E-01		3.10E-01
	C	45.7	1.32E+00		1.11E+00
	D	42.4	2.17E+01	2.35E+01	2.40E+01
	E	37.7	1.22E+02	1.72E+02	1.47E+02
	F	33.6		1.89E+02	1.76E+02
	G	31.3	1.39E+02	1.67E+02	1.38E+02
	H	30.0	2.01E+02	2.00E+02

Download these data

Protein purified on 11/7/2024 and assayed on 11/14/2014.
Data uploaded by Autumnbayerl and last updated on 2024-12-12 07:54:37.

Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.