D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409H

Expression Data

Yield (mg/mL): 0.11375

Kinetic Assay Data

**slope of W409H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.01E-08	7.12E-08	2.73E-08
B	25.00	4.92E-08	4.92E-08
C	8.33	4.92E-08		1.64E-08
D	2.78	2.19E-08	3.28E-08	1.26E-08
E	0.93	1.15E-08	1.64E-08	4.59E-09
F	0.31	4.37E-09		3.28E-09
G	0.10	1.64E-09		1.31E-09
H	0.03	1.26E-10	4.65E-11	1.53E-10

Yield (mg/mL): 0.11375 Dilution Factor: 10×

Protein purified on 10/23/24 and assayed on 10/30/24.
Data uploaded by catherinekaczowka and last updated on 2024-12-04 14:55:41.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.94E-01	1.79E-01	1.75E-01
B	25.00	1.39E-01	1.48E-01	1.43E-01
C	8.33	1.38E-01	1.21E-01	1.11E-01
D	2.78	6.85E-02	7.25E-02	8.18E-02
E	0.93	3.02E-02	3.41E-02	3.41E-02
F	0.31	1.12E-02	1.27E-02	1.20E-02
G	0.10	3.85E-03	3.95E-03	4.05E-03
H	0.03	1.55E-03	1.60E-03	1.90E-03

Yield (mg/mL): 3.25 Dilution Factor: 100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:47:32.

Design Data

Rosetta Score for W409H: 2.151

Citations

The unpublished data above were collected by students from Elmhurst University. For inquiries, please contact Sunshine Silver: <sunshine.silver@elmhurst.edu>.