D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H119D

Expression Data

Yield (mg/mL): 0.051

Kinetic Assay Data

**slope of H119D A_{420 nm} (s⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.67E-04	5.00E-04	1.00E-06
B	25.00	3.00E-04	3.00E-04
C	8.33	2.00E-04	2.33E-04
D	2.78	2.00E-04	2.00E-04
E	0.93	8.67E-05	8.00E-05
F	0.31	3.67E-05	3.00E-05
G	0.10	2.00E-05	1.00E-05	6.67E-07
H	0.03		1.17E-07	3.00E-07

Yield (mg/mL): 0.051 Dilution Factor: 10×

Protein purified on 10/24/2024 and assayed on 10/30/2024.
Data uploaded by fgeev3443 and last updated on 2024-12-04 14:56:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.94E-01	1.79E-01	1.75E-01
B	25.00	1.39E-01	1.48E-01	1.43E-01
C	8.33	1.38E-01	1.21E-01	1.11E-01
D	2.78	6.85E-02	7.25E-02	8.18E-02
E	0.93	3.02E-02	3.41E-02	3.41E-02
F	0.31	1.12E-02	1.27E-02	1.20E-02
G	0.10	3.85E-03	3.95E-03	4.05E-03
H	0.03	1.55E-03	1.60E-03	1.90E-03

Yield (mg/mL): 3.25 Dilution Factor: 100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:47:32.

Design Data

Rosetta Score for H119D: 1.638

Citations

The unpublished data above were collected by students from Elmhurst University. For inquiries, please contact Sunshine Silver: <sunshine.silver@elmhurst.edu>.