Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.1

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.37E-01	1.62E-01	1.57E-01
	B	25.00	1.29E-01	1.30E-01	1.40E-01
	C	8.33	1.17E-01	1.47E-01	1.29E-01
	D	2.78		7.69E-02	7.98E-02
	E	0.93		3.00E-02	3.08E-02
	F	0.31	7.95E-03	1.27E-02	1.09E-02
	G	0.10		3.75E-03	4.05E-03
	H	0.03		1.50E-04	1.50E-04

Yield (mg/mL): 1.1     Dilution Factor:  100×

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Protein purified on 10/30/2024 and assayed on 11/4/2024.
Data uploaded by pattycake and last updated on 2024-12-04 11:51:30.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00
	B	48.3	0.00E+00	0.00E+00
	C	45.7	0.00E+00	0.00E+00	0.00E+00
	D	42.4	7.00E-04	6.00E-04	8.00E-04
	E	37.7	2.65E-03	2.45E-03	2.75E-03
	F	33.6	2.50E-03	2.65E-03	2.75E-03
	G	31.3	2.75E-03	2.70E-03	2.80E-03
	H	30.0	2.20E-03	2.80E-03	2.75E-03

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Protein purified on 10/30/2024 and assayed on 11/9/2024.
Data uploaded by rhodosev and last updated on 2024-12-04 12:01:23.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.