Raw Data for BglB Variant N404S

Expression Data

SDS-PAGE
 0.425

Kinetic Assay Data

slope of N404S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0450 0.0400 0.0430
B 25.00 0.0275 0.0265 0.0290
C 8.33 0.0135 0.0130 0.0135
D 2.78 0.0055 0.0050 0.0055
E 0.93 0.00200 0.00150 0.00250
F 0.31 0.00050 0.00050 0.00050
G 0.10 0.000500 0.000500 0.000500
H 0.03 0.000000 0.000000
 0.425      10×

Protein purified on 9/22/2023 and assayed on 11/5/2023.
Data uploaded by Mer123 and last updated on 2024-12-03 14:50:29.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of N404S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00000 0.0000000
B 48.3 0.0005000 0.000500 0.0005000
C 45.7 0.001500 0.001500
D 42.4 0.006 0.006 0.006
E 37.7 0.0075 0.0075 0.0080
F 33.6 0.0080 0.0075
G 31.3 0.0070 0.0070 0.0075
H 30.0 0.0075 0.0065 0.0075

Protein purified on 9/22/2024 and assayed on 11/5/2023.
Data uploaded by Mer123 and last updated on 2024-12-03 15:10:43.


Reference Data for WT

slope of N404S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

4.579

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.