Raw Data for BglB Variant H119A

Expression Data

SDS-PAGE
 0.35

Kinetic Assay Data

slope of H119A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.45E-03 4.15E-03 4.35E-03
B 25.00 2.55E-03 2.35E-03 2.45E-03
C 8.33 1.45E-03 1.35E-03 1.30E-03
D 2.78 7.50E-04 5.50E-04 5.50E-04
E 0.93 2.50E-04 3.50E-04 4.00E-04
F 0.31 3.00E-04 1.50E-04
G 0.10 1.50E-04 0.00E+00 2.00E-04
H 0.03 2.00E-04 2.50E-04
 0.35      10×

Protein purified on 10/30/24 and assayed on 11/6/24.
Data uploaded by AndrewSIaia and last updated on 2024-12-04 11:49:19.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.37E-01 1.62E-01 1.57E-01
B 25.00 1.29E-01 1.30E-01 1.40E-01
C 8.33 1.17E-01 1.47E-01 1.29E-01
D 2.78 7.69E-02 7.98E-02
E 0.93 3.00E-02 3.08E-02
F 0.31 7.95E-03 1.27E-02 1.09E-02
G 0.10 3.75E-03 4.05E-03
H 0.03 1.50E-04 1.50E-04
 1.1      100×

Protein purified on 10/30/2024 and assayed on 11/4/2024.
Data uploaded by pattycake and last updated on 2024-12-04 11:51:30.


Thermostability Assay Data

Data missing or deleted from the database.


Reference Data for WT

slope of H119A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 0.00E+00 0.00E+00
C 45.7 0.00E+00 0.00E+00 0.00E+00
D 42.4 7.00E-04 6.00E-04 8.00E-04
E 37.7 2.65E-03 2.45E-03 2.75E-03
F 33.6 2.50E-03 2.65E-03 2.75E-03
G 31.3 2.75E-03 2.70E-03 2.80E-03
H 30.0 2.20E-03 2.80E-03 2.75E-03

Protein purified on 10/30/2024 and assayed on 11/9/2024.
Data uploaded by rhodosev and last updated on 2024-12-04 12:01:23.


Design Data

2.044

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.