Raw Data for BglB Variant D260E

Expression Data

SDS-PAGE
 0.16

Kinetic Assay Data

slope of D260E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.89E-02 2.59E-02 1.59E-02
B 25.00 1.54E-02 1.55E-02
C 8.33 1.22E-02 1.20E-02
D 2.78 6.45E-03 6.45E-03
E 0.93 3.40E-03 3.45E-03
F 0.31 1.45E-03 1.30E-03
G 0.10 5.50E-04 5.50E-04
H 0.03 0.00E+00 1.50E-04 2.50E-04
 0.16      100×

Protein purified on 10/30/2024 and assayed on 11/9/2024.
Data uploaded by JacobPrinzo14 and last updated on 2024-12-04 11:57:20.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.22E-01 2.15E-01 2.16E-01
B 25.00 1.76E-01 1.74E-01 1.69E-01
C 8.33 1.35E-01 1.36E-01 1.40E-01
D 2.78 8.66E-02 8.86E-02 8.99E-02
E 0.93 4.48E-02 4.22E-02 4.84E-02
F 0.31 1.72E-02 1.68E-02 1.70E-02
G 0.10 6.05E-03 5.50E-03 6.60E-03
H 0.03 0.00E+00 1.50E-04 2.00E-04
 1.8      100×

Protein purified on 11/4/2024 and assayed on 11/9/2024.
Data uploaded by kimj1282 and last updated on 2024-12-04 11:57:44.


Thermostability Assay Data

slope of D260E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 0.00E+00 1.50E-04 2.50E-04
C 45.7 0.00E+00 2.50E-04 1.50E-04
D 42.4 2.00E-04 2.00E-04
E 37.7 3.50E-04 3.00E-04 3.50E-04
F 33.6 9.50E-04 1.00E-03 9.50E-04
G 31.3 1.10E-03 1.10E-03 1.10E-03
H 30.0 1.25E-03 1.20E-03 1.25E-03

Protein purified on 11/4/24 and assayed on 11/9/24.
Data uploaded by bitaj and last updated on 2024-12-04 11:50:50.


Reference Data for WT

slope of D260E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 2.00E-04 2.00E-04
C 45.7 1.50E-04 3.50E-04
D 42.4 8.50E-04 9.50E-04
E 37.7 4.35E-03 4.75E-03 3.15E-03
F 33.6 4.35E-03 4.75E-03 4.10E-03
G 31.3 4.25E-03 4.75E-03 4.35E-03
H 30.0 4.45E-03 4.75E-03 4.45E-03

Protein purified on 10/30/2024 and assayed on 11/9/2024.
Data uploaded by Tanner and last updated on 2024-12-04 11:59:59.


Design Data

-0.828

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.