Raw Data for BglB Variant F289W

Expression Data

SDS-PAGE
 1.002

Kinetic Assay Data

slope of F289W A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.39E-01 1.39E-01 1.35E-01
B 25.00 6.78E-02 6.62E-02 6.57E-02
C 8.33 2.22E-02 2.33E-02 2.34E-02
D 2.78 8.55E-03 1.06E-02 1.06E-02
E 0.93 3.70E-03 3.75E-03 4.00E-03
F 0.31 1.30E-03 1.50E-03 1.35E-03
G 0.10 6.50E-04 6.50E-04 6.50E-04
H 0.03 0.00E+00 0.00E+00 0.00E+00
 1.002      10×

Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by zoyaahadian and last updated on 2024-12-05 12:11:52.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.37E-02 2.33E-02 2.15E-02
B 25.00 1.05E-02 1.01E-02 8.50E-03
C 8.33 3.35E-03 3.10E-03 3.30E-03
D 2.78 1.40E-03 1.50E-03 1.65E-03
E 0.93 8.50E-04 6.50E-04 6.00E-04
F 0.31 4.50E-04 3.50E-04 5.50E-04
G 0.10 2.50E-04 3.00E-04 3.50E-04
H 0.03 2.00E-04 2.00E-04 2.00E-04
 1.006      10×

Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by hananalhamami and last updated on 2024-12-05 12:43:58.


Thermostability Assay Data

slope of F289W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.00E-04 0.00E+00 2.00E-04
B 48.3 2.00E-04 2.00E-04
C 45.7 2.50E-04 2.50E-04
D 42.4 3.50E-04 4.50E-04 3.00E-04
E 37.7 2.15E-03 2.00E-03 2.15E-03
F 33.6 2.45E-03 2.55E-03 2.55E-03
G 31.3 2.75E-03 2.45E-03 2.50E-03
H 30.0 2.80E-03 2.50E-03 2.45E-03

Protein purified on 10/31/2024 and assayed on 11/9/2024.
Data uploaded by zoyaahadian and last updated on 2024-12-05 12:21:53.


Reference Data for WT

slope of F289W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 2.50E-04 2.50E-04
B 48.3 2.50E-04 2.00E-04
C 45.7 2.50E-04 2.00E-04
D 42.4 9.00E-04 9.00E-04 1.05E-03
E 37.7 3.50E-03 3.30E-03 3.70E-03
F 33.6 3.80E-03 3.70E-03 3.85E-03
G 31.3 3.80E-03 3.90E-03
H 30.0 4.15E-03 4.25E-03 4.30E-03

Protein purified on 10/31/2024 and assayed on 11/5/2024.
Data uploaded by hananalhamami and last updated on 2024-12-05 12:48:37.


Design Data

1.417

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.