D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P251D

Expression Data

Yield (mg/mL): 0.131

Kinetic Assay Data

**slope of P251D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.60E-03	2.85E-03	4.20E-03
B	25.00	3.15E-03	3.15E-03	2.55E-03
C	8.33	2.55E-03	2.85E-03	2.55E-03
D	2.78	1.80E-03	1.05E-03	1.50E-03
E	0.93	1.05E-03	1.05E-03	1.05E-03
F	0.31	7.50E-04	7.50E-04	6.00E-04
G	0.10		6.00E-04	4.50E-04
H	0.03		0	0

Yield (mg/mL): 0.031 Dilution Factor: 10×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by Nayelissanchez and last updated on 2024-11-22 13:52:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.07E-01	3.02E-01	2.72E-01
B	25.00	2.52E-01	2.48E-01	2.46E-01
C	8.33	2.34E-01	2.43E-01	2.25E-01
D	2.78	1.20E-01	1.32E-01	1.35E-01
E	0.93	5.48E-02	5.48E-02	5.48E-02
F	0.31	2.07E-02	2.12E-02	2.22E-02
G	0.10	7.50E-03	7.05E-03	7.35E-03
H	0.03	0	7.50E-04	4.50E-04

Yield (mg/mL): 4.121 Dilution Factor: 100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:08:38.

Design Data

Rosetta Score for P251D: 2.722

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.