D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P251N

Expression Data

Yield (mg/mL): 0.2041

Kinetic Assay Data

**slope of P251N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.30E-03		6.30E-03
B	25.00	4.95E-03		5.25E-03
C	8.33	4.50E-03		4.80E-03
D	2.78	3.60E-03	4.20E-03	3.75E-03
E	0.93	1.80E-03		1.65E-03
F	0.31	9.00E-04	1.35E-03	1.05E-03
G	0.10	9.00E-04	7.50E-04	7.50E-04
H	0.03		0	0

Yield (mg/mL): 0.2041 Dilution Factor: 10×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:39:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.09E-01	3.37E-01	3.18E-01
B	25.00	2.70E-01	2.71E-01	2.72E-01
C	8.33	2.25E-01	2.34E-01	2.33E-01
D	2.78	1.91E-01	1.93E-01	1.92E-01
E	0.93	7.59E-02	1.13E-01	8.67E-02
F	0.31	3.72E-02	3.69E-02	2.99E-02
G	0.10	1.47E-02	1.35E-02	1.19E-02
H	0.03	6.00E-04		6.00E-04

Yield (mg/mL): 5.032 Dilution Factor: 100×

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 14:39:33.

Thermostability Assay Data

**slope of P251N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	0	0
C	45.7	0	0
D	42.4	9.00E-04	6.00E-04	7.50E-04
E	37.7	1.20E-03	1.05E-03	1.05E-03
F	33.6	2.25E-03	2.25E-03	2.40E-03
G	31.3	2.25E-03	2.40E-03	2.40E-03
H	30.0	2.70E-03	2.40E-03	2.40E-03

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by VictoriaMansoor and last updated on 2024-11-22 13:54:31.

Design Data

Rosetta Score for P251N: 3.199

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.