Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.215

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		3.36E-01	3.75E-01
	B	25.00	2.97E-01	2.96E-01	3.02E-01
	C	8.33	2.74E-01	2.76E-01	2.77E-01
	D	2.78	1.63E-01	1.86E-01	1.64E-01
	E	0.93	6.99E-02	7.02E-02	8.04E-02
	F	0.31	3.14E-02	3.60E-02	2.96E-02
	G	0.10	1.01E-02	1.11E-02	1.35E-02
	H	0.03	0	0

Yield (mg/mL): 5.215     Dilution Factor:  100×

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by katherineg1230 and last updated on 2024-11-22 13:36:46.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0		0
	B	48.3	6.00E-04		6.00E-04
	C	45.7	6.00E-04	9.00E-04	7.50E-04
	D	42.4	1.65E-02	1.56E-02	1.61E-02
	E	37.7	1.91E-01	1.81E-01	1.90E-01
	F	33.6	2.26E-01	2.25E-01	2.12E-01
	G	31.3	1.83E-01	1.88E-01	1.75E-01
	H	30.0	1.94E-01	1.84E-01	1.78E-01

Download these data

Protein purified on 11/1/24 and assayed on 11/8/24.
Data uploaded by katherineg1230 and last updated on 2024-11-22 13:37:23.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.